Live imaging reveals the progenitors and cell dynamics of limb regeneration

eLIFE logo

Regeneration is a complex and dynamic process, mobilizing diverse cell types and remodelling tissues over long time periods. Tracking cell fate and behaviour during regeneration in active adult animals is especially challenging. Here, we establish continuous live imaging of leg regeneration at single-cell resolution in the crustacean Parhyale hawaiensis. By live recordings encompassing the first 4-5 days after amputation, we capture the cellular events that contribute to wound closure and morphogenesis of regenerating legs with unprecedented resolution and temporal detail. Using these recordings we are able to track cell lineages, to generate fate maps of the blastema and to identify the progenitors of regenerated epidermis. We find that there are no specialized stem cells for the epidermis. Most epidermal cells in the distal part of the leg stump proliferate, acquire new positional values and contribute to new segments in the regenerating leg.

Source: Abstract

Identification
Category
Document type
Research
ISBN/ISSN/NIPO
ISSN 2050-084X
IBIC classification
MBGR - Investigación médica
Bibliographic data
Place of publication
Cambridge, UK
Magazine
eLife Sciences
En línea
En línea
Retrieved
Year
2016
Month
October
Language
English
License
Creative Commons
Additional Information
Source
AT Observatory